Institut penyelidikan saintifik yang besar

1. Project Overview

A large scientific research institute—comprising multiple departments such as molecular biology, nanotechnology, advanced materials, and pharmaceutical sciences—initiated a major infrastructure upgrade to centralize its high‑containment and high‑sensitivity research capabilities. The institute required a flexible, scalable cleanroom facility capable of supporting diverse research activities ranging from cell culture and viral vector production to nanofabrication and analytical chemistry.

Unlike a single‑owner pharmaceutical company, the institute hosts dozens of independent research groups, each with varying cleanliness, safety, and equipment requirements. The primary challenge was to design a shared cleanroom equipment ecosystem that could serve multiple users without cross‑interference, while maintaining compliance with institutional biosafety levels (BSL‑2 and BSL‑3) and ISO 14644 standards.

2. Client Requirements

• Fleksibiliti pelbagai pengguna:Perkhidmatan: Zon bilik bersih yang boleh dikonfigurasi semula untuk protokol penyelidikan yang berbeza (contohnya, kerja sel steril seminggu, sintesis nanopartikel seterusnya).

• Segregated Containment: Isolated modules for BSL‑2, BSL‑3, and low‑humidity nanofabrication processes, each with dedicated HVAC and airlock systems.

• Kebolehpercayaan & amp; Masa aktif:Perkhidmatan: Operasi 24/7/365 dengan peralatan bilik bersih berlebihan untuk mengelakkan masa henti penyelidikan.

• Energy Efficiency: Given the large footprint (3,500 m²), the institute demanded low‑energy cleanroom HVAC and filtration solutions.

• Latihan & Akses: Equipment interfaces designed for easy operation by rotating students, postdocs, and visiting scientists.

3. Cleanroom Equipment Engineering Solution

Our engineering team delivered a centralized cleanroom equipment package tailored to a multi‑tenant research environment:

A. Zoned HVAC and Filtration

• Dedicated AHU per cleanroom class:Perkhidmatan: Unit pengendalian udara berasingan untuk ISO  Class 5, Kelas  6, dan kelas  7 kawasan.

• HEPA/ULPA filter banks: Ceiling‑mounted filter modules with adjustable airflow for research‑grade unidirectional or non‑unidirectional patterns.

• Low‑velocity displacement ventilation in low‑turbulence zones (e.g., for electron microscopy prep).

B. Modular Cleanroom Architecture

• Panel dinding keras yang boleh dibuang:Perkhidmatan: Membolehkan konfigurasi semula susun atur makmal tanpa pengubahsuaian struktur.

• Color‑coded modular walls: Visual differentiation of cleanliness levels (e.g., white = ISO 7, blue = ISO 6, yellow = BSL‑3).

• Interlocked pass‑through autoclaves and chemical dunk tanks: For safe material transfer between containment levels.

C. Shared Containment and Process Equipment

• Multiple Class II Type A2 biosafety cabinets (BSCs): Each equipped with individual monitoring but connected to a common exhaust plenum.

• Portable glovebox isolators: For oxygen/moisture‑sensitive materials research.

• Centralized vacuum and gas systems: Ultra‑high purity nitrogen, argon, and clean dry air distributed via stainless steel manifolds.

D. Central Monitoring & Access Control

• Room‑by‑room EMS/BMS: Real‑time pressure, particle count, temp, RH, and door interlock status.

• RFID‑based access system: Restricts users to authorized cleanroom classes and records usage for billing/accountability.

• Visual alarm beacons at each entry for rapid pressure‑cascade failure notification.

4. Key Engineering Challenges & Solutions

5. Validation & Compliance

Semua peralatan bilik bersih layak untuk & nbsp;ISO 14644‑1:2015Perkhidmatan: Keperluan Keselamatan Biologi:

• DQ: Simulated multi‑user scenarios (e.g., simultaneous cell culture and nanoparticle synthesis).

• IQ:Perkhidmatan: Sambungan utiliti yang disahkan, pensijilan penapis, dan pemasangan peralatan.

• OQ: Tested air change rates (20–60 ACH depending on class), pressure cascades, and recovery time (<15 min to ISO Class 5).

• PQ:Perkhidmatan: Tiga bulan pemantauan dinamik dengan protokol penyelidikan sebenar, termasuk pengambilan sampel udara mikroba.

Outcome: The institute achieved full operational certification with zero cross‑contamination events during the validation period.

6. Project Outcome

• Kawasan bilik bersih:Perkhidmatan: 3,500  m² (ISO 5: 400  m² , ISO 6: 1,200  m² , ISO 7: 1,900  m²)

• Kapasiti Pengguna:Perkhidmatan: Sehingga 150 penyelidik sehari di 28 modul makmal bebas

• Penjimatan Tenaga:Perkhidmatan: 32% pengurangan tenaga HVAC melalui kipas kelajuan berubah-ubah dan pengudaraan yang dikawal permintaan

• Kadar Penggunaan:Perkhidmatan: Maklumat 85% dalam tempoh enam bulan pembukaan, menampung 12 kumpulan penyelidikan dari 4 fakulti

Institut Penyelidikan kini mengendalikan a kemudahan teras bilik bersih yang benar-benar berkongsi dan berprestasi tinggimembolehkan projek lintas disiplin yang sebelum ini mustahil disebabkan oleh pencemaran atau konflik pengendalian.

7. Kesimpulan

Untuk institut penyelidikan saintifik yang besar, kejuruteraan peralatan bilik bersih mesti mengutamakan fleksibiliti, pemisahan, dan reka bentuk yang berpusat pada penggunaPerkhidmatan: jauh lebih daripada dalam kemudahan farmaseutikal khusus. Projek ini menunjukkan bahawa dengan HVAC zon, seni bina dinding keras modular, dan pemantauan berpusat, kompleks bilik bersih tunggal boleh dengan selamat berkhidmat kepada beratus-ratus penyelidik di seluruh disiplin BSL-2, BSL-3, dan nanofabrication. sambil mengekalkan ISO  Class 5 Integriti dan kecekapan tenaga.